Gregory Bonito

Gregory Bonito

Fungal Systematist

Gregory Bonito’s research uses molecular sequence data and morphology to investigate questions pertaining to the evolution, systematics, ecology and biogeography of fungi. Gregory has a strong interest in truffles, fungi that sequester their spores in fruiting bodies below ground. Together with the staff at the Royal Botanic Gardens (RBG) Melbourne, Gregory is working on the systematics and biogeography of the mushroom genera Austropaxillus, Austroboletus and the related truffle genus Gymnopaxillus to investigate evolutionary patterns related to below-ground transitions to the truffle form. Gregory’s research also pertains to rhizosphere interactions between fungi and plants, fungal genomics, and the evolution of bacterial endosymbionts in early diverging terrestrial fungi.

Gregory’s research at the RBG will utilise and develop the RBG's herbarium collections, with an overarching aim to integrate systematics and taxonomic research into practical tools, including applications in the areas of fungal identification (DNA barcoding), biodiversity inventory and conservation biology. 

  • Refereed journal articles
  • Books
  • Book chapters
  • Other

Berch, S. and Bonito, G. (2014). Cultivation of Tuber melanosporum and T. aestivum (Tuberaceae) in British Columbia, Canada. Mycorrhiza 10.1007/s00572-014-0562

Bonito, G., Reynolds, H., Hodkinson, B., Nelson, J., Tuskan, G., Robeson, M., Schadt, C. and Vilgalys, R. (2014). Plant host and soil origin influence fungal and bacterial assemblages in the rhizosphere of woody plants. Molecular Ecology. DOI: 10.1111/mec.12821

Benucci, G.M.N., Bonito, G., Baciarelli Falini, L., Donnini, D. and Bencivenga, M. (2013). Mycorrhizal inoculation of pecan seedlings with some marketable truffles. Acta Mycologica 47, 179–184.

Bonito, G., Smith, M., Nowak, M., Healy, R., Guevara, G., Cázares, E., Kinoshita, A., Nouhra, E., Domínguez, L., Tedersoo, L., Murat, C., Wang, Y., Moreno, B., Pfister, D., Nara, K., Zambonelli, A., Trappe, J. and Vilgalys, R. (2013). Historical biogeography and diversification of truffles in the Tuberaceae and its newly identified sister lineage. PLoS ONE 8, e52765. doi:10.1371/journal.pone.0052765.

Guevara, G., Bonito, G., Trappe, J., Cázares, E., Williams, G., Healy, R., Schadt, C. and Vilgalys, R. (2013). New North American truffles (Tuber spp.) and their ectomycorrhizal associations. Mycologia 105, 194–209. doi:10.3852/12-087.

Healy, R., Smith, M.E., Bonito, G., Pfister, D.H., Ge, Z-W., Guevara, G., Williams, G., Stafford, K., Kumar, L., Lee, T., Hobart, C., Trappe, J., Vilgalys, R. and McLaughlin, D. (2013). High diversity and widespread occurrence of mitotic spore mats in ectomycorrhizal Pezizales. Molecular Ecology 22,1717–1732. doi: 10.1111/mec.12135.

Husbands, D., Henkel, T., Bonito, G., Vilgalys, V. and Smith, M. (2013). New species of Xerocomus (Boletales) from the Guiana Shield, with notes on their mycorrhizal status and fruiting occurrence. Mycologia 105, 422–435. doi: 10.3852/12-146.

Shakya, M., Gottel, N., Castro, H., Yang, Z., Gunter, L., Labbé, J., Muchero, W., Bonito, G., Vilgalys, R., Tuskan, G., Podar, M. and Schadt, C. (2013). A multifactor analysis of fungal and bacterial community structure in the root microbiome of mature Populus deltoides trees. PlosONE 8, e76382. doi: 10.1371/journal.pone.0076382.

Smith, M.E., Gryganskyi, A., Bonito, G., Nouhra, E., Moreno-Arroyo, B. and Benny, G. (2013). Phylogenetic analysis of the genus Modicella reveals an independent evolutionary origin of sporocarp-forming fungi in the Mortierellales. Fungal Genetics and Biology doi: 10.1016/j.fgb.2013.10.001.

Wang, X.H., Benucci, G.M.N., Xie, X.D., Bonito, G., Leisola, M., Liu, P.G. and Shamekh, S. (2013). Morphological, mycorrhizal and molecular characterization of Finnish truffles belonging to the Tuber anniae species-complex. Fungal Ecology 6, 269–280.

Benucci, G., Bonito, G., Baciarelli Falini, L. and Bencivenga, M. (2012). Mycorrhization of Pecan trees (Carya illinoinensis) with commercial truffle species: Tuber aestivum Vittad. and Tuber borchii Vittad. Mycorrhiza. Mycologia 22, 383–392. doi:10.1007/s00572-011-0413-z.

Bonito, G., Smith, M., Brenneman, T. and Vilgalys, R. (2012). Assessing ectomycorrhizal fungal spore banks of truffle producing soils with pecan seedling trap-plants. Plant and Soil 356, 357–366. doi:10.1007/s11104-012-1127-5.

Bonito, G., Brenneman, T. and Vilgalys, R. (2011). Ectomycorrhizal fungal diversity in orchards of cultivated pecan (Carya illinoinensis; Juglandaceae). Mycorrhiza 21, 601–612.

Bonito, G., Brenneman, T. and Vilgalys, R. (2011) Tuber ectomycorrhizae on Pecan trees (Carya illinoinensis; Juglandaceae). Proceedings from the 3rd International Congress on Tuber. Spoleto, Italy. Acta Botanica Yunnanica Supp. XVI: 39–51.

Bonito, G., Trappe, J., Donovan, S. and Vilgalys, R. (2011). The Asian black truffle Tuber indicum can form ectomycorrhizae with North American host plants and can complete its life cycle in non-native soils. Fungal Ecology 4, 83–93. doi: 10.1016/j.funeco.2010.08.003

Gryganskyi, A.P., Lee, S.C., Litvintseva, A.P., Smith, M.E., Bonito, G., Porter, T.M., Anishchenko, I.M., Heitman, J. and Vilgalys, R. (2011). Structure, function, and phylogeny of the mating locus in the Rhizopus oryzae complex. PLoS ONE 5, e15273. doi:10.1371/journal.pone.0015273

Tedersoo, L., Abarenkov, K., Nilsson, H., Schüβler, A., Grelet, G., Kohout, P., Oja, J., Bonito, G., Veldre, V., Jairus, T., Ryberg, M., Larsson, K. and Kõljalg, U. (2011). Tidying up International Nucleotide Sequence Databases (INSD): ecological, geographical and sequence quality annotation of ITS sequences of mycorrhizal fungi. PLoS ONE 6, e24940.

Bonito, G., Gryganskyi, A., Vilgalys, R. and Trappe, J. (2010). A global meta-analysis of Tuber ITS rDNA sequences: species diversity, host specificity, and long-distance dispersal. Molecular Ecology 19, 4994–5008. doi: 10.1111/j.1365-294X.2010.04855.x

Bonito, G., Isikhuemhen, O. and Vilgalys, R. (2010). Identification of fungi associated with municipal compost using DNA-based techniques. Bioresource Technology 101, 1021–1027.

Bonito, G., Trappe, J., Rawlinson, P. and Vilgalys, R. (2010). Improved resolution of major clades within Tuber and taxonomy of species within the Tuber gibbosum complex. Mycologia 101, 1042–1057.

Trappe, M., Trappe, J. and Bonito, G. (2010). Kalapuya brunnea gen. & sp. nov. and its relationship to the other sequestrate genera in Morchellaceae. Mycologia 101, 1058–1065.

Bonito, G. (2009). Fast DNA-based identification of the black truffle Tuber melanosporum using direct PCR and species-specific primers. FEMS Microbiology Letters 301, 171–175.

Bonito, G., Trappe, J. and Vilgalys, R. (2009). North American truffles in the Tuberaceae: molecular and morphological perspectives. Proceedings of the 5th International Workshop on Edible Mycorrhizal Mushrooms. Chuxiong, China. Acta Botanica Yunnanica. Supplement XVI.

Healy, R., Bonito, G. and Guevara, G. (2009). Pachyphloeus marronius, a new truffle species from Iowa and Mexico. Mycotaxon 107, 61–71.

Healy, R., Bonito, G. and Trappe, J. (2009). Calongea, a new genus of hypogeous Pezizaceae. Anales del Jardin Botanico de Madrid 66, 25–32.

Schoch. C. and 63 other authors. (2009). The Ascomycota tree of life: a phylum wide phylogeny clarifies the origin and evolution of fundamental reproductive and ecological traits. Systematic Biology 58, 224–239.

Guevara, G., Bonito, G., Cázares, E., Rodríguez, J., Vilgalys, R. and Trappe, J. (2008). A new species of black truffle from Mexico, Tuber regimontanum. Mexican Journal of Mycology 26, 17–20.

Iotti, M., Amicucci, A., Bonito, G., Bonuso, E., Stocchi, V. and Zambonelli, A. (2007). Selection of a set of specific primers for the identification of Tuber rufum: a truffle species with high genetic variability. FEMS Microbiology Letters 277, 223–231.

Collins, S.L., Bettencourt, L.M.A., Hagberg, A., Larkey, L., Brown, R.F., Moore, D.I., Bonito, G.M., Delin, K.A., Jackson, S.P., Johnson, D.W., Burleigh, S.C., Woodrow, R.R. and McAuley, J.M. (2006). New opportunities in ecological sensing using wireless sensor networks. Frontiers in Ecology and the Environment 4, 402–407.

Bonito, G.M, Coleman, D.C., Haines, B.L. and Cabrera, M.L. (2003). Can nitrogen budgets explain differences in soil nitrogen mineralization rates between Oak Pine and Northern Hardwood stands? Forest Ecology and Management 276, 563–574.

Zambonelli, A. and Bonito, G. (eds) (2013). Edible ectomycorrhizal mushrooms: current knowledge and future prospects. Soil Biology Series: 34. Springer, New York.

Payen, T., Murat, C., Bonito, G. (2014). Truffle phylogenomics: new insights into truffle evolution and truffle life cycle. In F. Martin (ed.), Advances in Botanical Research, vol. 70: Fungi, pp. 211–234. Academic Press.

Benucci, G.N., Bonito, G., Donnini, D., Baciarelli Falini, L., Di Massimo, G. and Bencivenga, M. (2013). Truffles, timber, food, and fuel: possibilities in multi-cropping truffles with economically important plants. In A. Zambonelli and G. Bonito (eds), Edible ectomycorrhizal mushrooms: current knowledge and future prospects, Soil Biology Series: 34, pp. 265–280. Springer, New York.

Martin, F. and Bonito, G. (2013). Ten years of genomics for ectomycorrhizal fungi: what have we achieved and where are we heading? In A. Zambonelli and G. Bonito (eds), Edible ectomycorrhizal mushrooms: current knowledge and future prospects, Soil Biology Series: 34, pp. 383–402. Springer, New York.

Smith, M. and Bonito, G. (2013). Systematics and ecology of ectomycorrhizal mushrooms. In A. Zambonelli and G. Bonito (eds), Edible ectomycorrhizal mushrooms: current knowledge and future prospects, Soil Biology Series: 34, pp. 17–40. Springer, New York.

Pregenzer, A., Parmenter, R.R., Passell, H., Vande Castle, J., Budge, T. and Bonito, G. (2004). Sustainability in arid grasslands: new technology applications for management. In M. Shachak, S.T.A. Pickett, J.R. Gosz and A. Perevolotski (eds), Biodiversity in drylands, pp. 250–265. Oxford University Press, New York.

Estrin, D., Michener, W., Bonito, G. and the workshop participants (2003). Environmental cyberinfrastructure needs for distributed sensor networks: a report from a National Science Foundation sponsored workshop. Scripps Institution of Oceanography, La Jolla, CA, August 12–14, 2003.

Nagel, D., Hughes, D. and Bonito, G. (2003). Environmental sensor networks. In Any Signal, Any Scale, Anywhere: Multi-sector Crisis Management Consortium – conference proceedings. Arlington, VA, Jan 11–13, 2002.

Bonito, G. and Michner, W. (2002). In-situ environmental sensor technology highlights. In Proceedings of the 2002 National Conference on Environmental Science and Technology. Greensboro, North Carolina, September 8–10, 2002, pp. 185–194. Battelle Press.

Goodin, D. and Bonito, G. (2002). In situ sensors. In Scalable Information Networks for the Environment conference proceedings. San Diego Supercomputer Center, October 29–31, 2001.  

Weisenberger, A., Bonito, G., Reid, C. and Smith, M. (2013). Method and apparatus to image biological interactions in plants. Invention disclosure #1343.

Sharma, J., Trela, B., Wang, S., Smith, M. and Bonito, G. (2012). Pecan truffle (Tuber lyonii) in Texas. Pecan South Magazine Fall, 19–24.

Smith, M., Bonito, G., Sharma, J., Long, J., Long, B. and Brenneman, T. (2012). Pecan truffles (Tuber lyonii): what we know and what we need to know. Georgia Pecan Magazine Spring, 52–59.

Le Tacon, F., Wang, Y.W., Jeandroz, S., Bonito, G. and Murat, C. (2009). Phylogénie et paléogéographie des truffes. Société d'horticulture et d'histoire naturelle de l'Hérault.

Bonito, G., Vilgalys, R. and Isikhuemhen, O. (2008). Second annual truffle biotechnology workshop. Inoculum59(5), 20.

Bonito, G. and Casarez, E. (2007). Truffling, south of the border. Inoculum. 58(6), 31.

Michener, W. and Bonito, G. (2003). Ecoinformatics training for field stations. The LTER Network Newsletter 16(1),10.

Bonito, G. (2001). An in-situ environmental sensor resource.

Bonito, G., Porter, D. and Davis, D. (2001). Mushrooms and Fungi at the Georgia State Botanical Garden, Athens, GA. (Brochure)